Effective for both frozen and paraffin-embedded tissue sections.
Specific protocols for use, including blocking with non-fat milk and incubation times, can be found in the technical datasheet provided by Santa Cruz Biotechnology. Why is it in a .rar file? sc25336-dcupd104-rar
Typically used at dilutions ranging from 1:100 to 1:1,000. Users have reported success at much higher dilutions, such as 1:8,000, depending on sample quality. such as 1:8
The "sc-25336" identifier refers specifically to a monoclonal mouse antibody used for detecting the vinculin protein in mouse, rat, and human samples. Key Information about sc-25336 sc25336-dcupd104-rar
Information regarding the chemical composition and safety handling of the antibody solution.
Detailed protocol information and reactivity profiles.