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With Castiel’s blood already secured, the brothers turn to Crowley. He agrees to help but demands they find the first, as his own blood is a "last resort" that could be used against him.
While Sam and Dean have two of the three blood samples, they discover Bobby has vanished, and their only remaining ally, Crowley , is now in the enemy's hands.
Dick Roman summons Crowley into a devil’s trap to prevent him from helping the Winchesters. [S7E22] There Will Be Blood
Frustrated by being left behind in a safe (made of iron to contain him), Bobby manages to possess a motel maid to retrieve his flask and pursue Dick Roman on his own. This act confirms Sam's fears that Bobby is becoming a dangerous, vengeful spirit. Setting the Finale Stage
His presence is marked by icy mirrors and flickering lights. With Castiel’s blood already secured, the brothers turn
In the penultimate episode of Season 7, Sam and Dean Winchester race to assemble a powerful weapon to take down Dick Roman and the Leviathans. Following instructions from the Word of God tablet, they must combine the bone of a righteous human with the blood of three "fallen" beings: a fallen angel, the King of Hell, and an Alpha monster. The Quest for the Alpha's Blood
The Leviathan Edgar arrives to kill the Alpha and "purge" the vampire race. After a tense struggle, Sam decapitates Edgar with the brothers' new "vamptonite"—blood taken from a corn-syrup-dazed human—giving the Alpha a change of heart. Grateful but still hostile, the Alpha hands over his blood and releases a captive boy, Allan, to the Winchesters. Bobby’s Descent Dick Roman summons Crowley into a devil’s trap
The boys track the Alpha Vampire to a hidden monastery. They attempt to negotiate, warning him that the Leviathans—his supposed allies—are actually planning to exterminate his kind by poisoning their food source.